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Histopathology

Totally immerse immediately each specimen in a tightly secured container with 10% neutral buffered formalin
Use a separate container for each specimen
Do not crush the specimen with forceps, hemostats, or other instruments. Cautery will cause heat artifact.
DO NOT FREEZE the specimens
Do not force a large specimen into a small container. Formalin volume to specimen ratio should be 10:1
Label each container (not the lid) with patient’s name and source of specimen
Complete a Histopathology test requisition and send with specimen(s). Only one Histopathology test requisition is needed per patient
Each container and specimen must be separately identified on the test requisition
The test requisition should contain patient’s date of birth, sex, clinical information and anatomic source of tissue
Specimen Handling For Immunohistochemistry (IHC):
Submit formalin fixed paraffin embedded blocks/4m sections on coated glass slides. Preferably submit one routinely stained H & E section of the block with appropriate clinical history and copy of Histopathology report
Alternatively submit specimens as for Routine Histopathology
Unstained FNA smears are also acceptable. Submit 2 smears for each marker requested
Specimen Handling For Direct Immunofluorescence (DIF):
Submit minimum 3 mm punch biopsy of Skin or Conjunctival specimen in Phosphate buffered normal saline OR Michel’s fluid available from LPL
Submit a linear 1 cm tissue core of Kidney biopsy for DIF in buffered normal saline or Michel’s fluid.
Ship refrigerated.
Immunofluoresence cannot be performed on formalin fixed tissues
Attach relevant clinical history
Specimen Handling For Special Stains:
Source and specific site (left, right, quadrant, etc.)
Nature of lesion (solid/cystic, mobile/fixed, functional/nonfunctional, etc.)
Any other pertinent history (previous surgery, presence of other masses, previous abnormal findings)
Mammographic, X-ray or other radiologic findings
Specimen Collection, Non-Gynecologic
Submit specimens as for Routine Histopathology or submit formalin fixed paraffin blocks. Attach Histopathology report, relevant clinical history and one Histology section of the block submitted
Specimen Handling For DNA Ploidy and S Phase:
DNA Histograms are performed by Flow Cytometry on Paraffin embedded tissues or tissues submitted as for Routine Histopathology
Submit copy of the Histopathology report with relevant clinical details

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